核酸探针 meaning in Chinese
nucleic-acid probe
Examples
- Nonradioactive nucleic probe to tgev was prepared and was applicated to detect tgev at first stage , which established foundational work for differentiating tgev from prcv and investigation on epidemiology of tgev
制备了tgev非放射性标记的核酸探针，并初步应用于检测tgev 。二者为tgev鉴别诊断、血清学调查奠定了基础。 - The specificity of the nucleic acid probe was very strict . lt reacted positively with iltv dna only and it react negatively with the nuleic acid of ndv , bv and ibdv . the sensitivity of this kind of probe is very high . ilt could even detect 20pg ' s iltv dna
结果表明：该种核酸探针具有高度的特异性，它仅与iltv的dna呈现阳性反应，而与新城疫病毒、传染性法氏囊病病毒和传染性支气管炎病毒的核酸等均呈阴性反应。该种探针具有高度的敏感性，能够检测到20pg的iltv的dna 。 - Lastly by using the technique of dot blot hybridization , the genome dna of chlamydia was detected with the probe of momp gene labeled with dig - 11 - dutp by using the way of random primer . the results showed the degree of sensitivity of the probe was 10 pg and other pathogens could not be detected by this probe . by comparing the diagnostic ways of nucleotide probe and fc , the technique of nucleotide probe were proved to have high sensitivity and speci fi city
最后，用地高辛随机引物法标记成momp基因核酸探针，斑点杂交检测衣原体基因组dna ，灵敏度可达10pg ，且不能检出其它病原体的核酸。将核酸探针法与补体结合反应法对衣原体感染的诊断进行比较，初步证明该探针具有较高的敏感性与较强的特异性。 - The author reviewed the detection measures of prunus necrotic ringspot virus , and related the research progression of the pathogen detection technology inside and outside . the template amplication technology include pcr assays ^ nasba and so on . the cdna and crna probe which labeled with the isotope % biotin or dig , the offset probe and the peptide probe can be applied to magnify the signal . pcr - gene scan assays and pcr agilent chip chamber combine the template amplication and the signal magnification
本文回顾了李坏死环斑病毒的检测方法，较全面地评述了国内外病原物检测技术研究进展：在模板的扩增有各种pcr技术、 nasba技术；在信号的放大有同位素、生物素或dig标记的cdna和crna探针，分支探针和肽核酸探针；模板扩增和信号放大相结合的有pcr -基因扫描技术、 pcr安捷伦芯片实验室技术；模板扩增和杂交以及信号放大相结合的有pcr - elisa技术、实时荧光pcr技术、生物芯片技术。 - One was cloning , sequence analysis and expression of the fragment containing the b and c antigenic sites locating at the 5 " terminus in spike gene of tgev in prokaryotic expression system ( fused with gst ) , the other was preparation of non - radioactive probe labeled by digoxigenin for detecting the rna extracted from tgev by assay of dot - blot
为了鉴别诊断tgev与prcv及对tgev进行流行病学调查，本研究采用原核表达系统( gst融合表达系统)表达tgev纤突蛋白( s蛋白)中含有b和c抗原位点的多肽，并且制备了非放射性地高辛标记的核酸探针，通过斑点杂交( dot - blot )检测tgev核酸rna 。