杂交地带 meaning in Chinese

hybrid zone

2 . 5 ul of 10 x reaction buffer , 1 . 5 ul 25mm mgcl2 , 0 . 3 ul lomm dntp , 0 . 5 ul taqdna polymerase ( 5 u / ul ) , and lul ( = 20ng ) of primer were used in per reaction . each reaction was overlaid with one drop of paraffin oil . the initial denaturation step was used at 94 for 1 min 45 sec ; then denatured at 94 for 30 sec , annealed at 37 1 min , extended at iv b 72 for 2 min and repeated the cycle 45 times , at last , extended at 72 ' c for lomin
等( 1995 )利用rapd标记区分美国东部一杂交地带的蟋蟀的两个姐妹种， allonemobiusfasciatus和a . socius ，并于1998年使用rapd和异型酶标ic做出了这两种蟋蟀的基因连锁图；国内田英芳、郑哲民（ 20of ）首次将rapd技术运用于蟋蟀总科的分子系统学研究中，采用2种引物对7种蟋蟀进行了基困组dna多态性研究，并应用upg问a法构建树状图，椎测系统发生关系