早期启动子 meaning in Chinese
early promoter
Examples
- In this study , a 1 . 7kb kpni fragment and a lacz gene expression cassette carrying the e . coli lacz gene under the control of sv40 promoter were inserted into the transfer vector pbdtk - uni ( a 277bp acci - accl fragment in the tk gene was deleted ) . the new transfer vector was called puni - lacz . the transfer vector puni - lacz and purified genomic dna of strain bartha - k61 were used to cotransfect vero cells using lipofectin transfection procedure
本研究以呈ge ~ -表型的经典弱毒疫苗bartha - k61株为亲本株，在通用prv转移载体pbdtk - uni的基础上，在其多克隆位点中插入由sv40早期启动子控制下的lacz基因表达盒，同时将下游同源臂增加了一个1 . 7kb的kpni片段，使上下游同源臂的长度都超过了1kb ，构建了一个新的转移载体puni - lacz 。 - The expression efficiency difference between ped5 and pcdhfrl , a vector utilizing cmv enhancer / promoter ( pcmv - ie ) for foreign protein production , was analyzed using human interferon - p ( ifn - ) gene and human secreted alkaline phosphatase ( seap ) gene as reporters . when analyzed in transient expression , ped5 showed a little more protein produciton than pcdhfrl . however , in continuous expression , when serum concentration was lessened to slow down cell proliferation , ped5 expressed 3 . 1 times more reporter proteins than pcdhfrl , which implied that pef - io was less affected by cell cycle status in contrast to pcmv - ie , making ped5 a good expression vector for foreign protein production
应用人-干扰素( ifn - )和人分泌型碱性磷酸酶( seap )基因作为报告基因，对含有巨细胞病毒即早期启动子( p _ ( cmv - ie ) )的表达载体pcdhfr1和ped5表达外源蛋白的能力进行了比较，发现对于瞬时表达， ped5略好于pcdhfr1 ；在稳定表达中，通过降低血清浓度，使细胞增殖缓慢，这时ped5表达外源蛋白的能力较pcdhfr1高3 . 1倍。