文库构建 meaning in Chinese
library construction
Examples
- Construction of araneus ventricosus major ampullate gland cdna library and screening spider silk gene
文库构建和丝蛋白基因筛选 - In the process of library construction , some technical details were studied , such as the combination of dna silver staining with rd technique , purification of pcr product , storage of the data about probes and bioinformatics analysis of one est , etc . 2
在文库构建过程中，对其中的一些技术细节，如在dna银染与rd技术结合、 pcr产物纯化、探针数据的管理、 est的生物信息学分析等方面进行了探索。 - Constructing cdna expressing library of erythrocytic plasmodium falciparum from hainan : the total rna was obtained by using . triplix kit . a modified oligo ( dt ) primer ( cds ffi pcr primer ) was used in the single - stranded ( ss ) dna synthesis reaction . the ss - dna was reversely transcripted from total rna . double - stranded ( ds ) cdna was amplified by long - distance ( ld ) pcrafter the digestion with proteinase k and sfi i , the cdna with no less than 200bp was collected and purified by glass - milk kitthe library was constructed after the ligation of cdna to tiplex2 phage particle packaged with the packaging extract system in vitro . a high titer and high recombinant ratio of cdna library was constructed
构建恶性疟原虫海南株红内期cdna表达文库提取红内期恶性疟原虫海南株总rna ，直接以总rna为模板使用cdna文库构建试剂盒，首先反转录合成ss一dna ，再扩增合成ds一dna ( cdna ) ，对扩增产物用蛋白酶k消化及左z丁i酶切，抽提蛋白、去除rna后，用玻璃奶试剂盒纯化、回收20obp以上的片断，经与载体连接再用蛋白包装物包装后形成未扩增文库，最后扩增完成恶性疟原虫海南株红内期cdna表达文库的构建。