整合载体 meaning in Chinese

integrating vector

The artificial antibacterial peptide - ceme gene was designed according to the codon with the highest frequency in pichia pastoris , then the ceme gene was integrated into the chromosome of pichia pastoris strain - gsl 15 by electroporation
我们根据人工设计的新型抗菌肽ceme序列设计ceme基因，重组到酵母胞内表达型的整合载体phild2中，通过电转化作用将ceme基因整合至酵母宿主gsll5染色体上。
Pcr products were inserted into pbluescriptsk using psti and xhoi . the element bearing resistance to streptomycin ( sm ) and spectinomycin ( sp ) was excised from php45 and inserted into gene coding region . the disrupted gene was isolated as a pstl and xhol fragment and cloned into prl271
首先将pcr扩增片段( 1 . 5kb )利用pst和xho插入常规克隆载体pbluescriptsk ，利用基因内部合适的酶切位点插入sp sm抗性片段，再利用pst和xho位点将已插入抗性基因的pcr片段，转入整合载体prl271中获得体外基因中断，最后利用三亲本杂交获得体内突变。