插入失活 meaning in Chinese
insertional inactivation
Examples
- Inactivation of genes which involved in signal transduction and analyze the phenotypes of mutants all the mutants in this study were inactivated by inserting a resistance gene
信号转导相关基因突变体的构建及表型分析本研究全部通过插入失活获得突变体。 - Based on a 3 . 1kb pst i fragment of genomic dna of a wild s . avermitilis , a 1 . 5 kb apramycin resistance fragment was inserted into sph i site of avec gene in the 3 . 1 kb fragment , then a recombinant plasmid pc05 was obtained by introducing above inactivated avec fragment into mcs region of phjl401 . competent cells of et12567 were transformed by recombinant plasmid pid03 and pc05 respectively
以含有avec基因的3 . 1kb基因组dnapsti片段为基础，将1 . 5kb的安普霉素抗性基因片段插入到avec基因中的sphi酶切位点，再将此插入失活的avec基因片段连接到具有接合转移功能(含有orit基因)的链霉菌-大肠杆菌穿梭质粒phjl401的多克隆位点区，由此得到重组质粒pc05 。 - The tn5 inserted in the orf4 . it was demonstrated that this fragment was related to magnetosome biosynthesis by experiment of functional complement . the orf4 encodes a protein which is homologous to the atpase from magnetospirillum magnetotacticum ms - 1 and contains a rec domain which has a chey - homologous receiver domain
该片段由6个orf组成， tn5插入在3621bp - 3622bp间，位于orf4起始密码子下游的84bp处， orf4因tn5插入失活，通过功能互补实验证明该片段与磁小体合成相关。