接种密度 meaning in Chinese
inoculum density
Examples
- Round shaped primmorphs of less than 100 mm were formed 3 days after transferring the dissociated cells into seawater containing ca2 + and mg2 +
发现细胞接种密度,组织块的健康状况,培养温度对海绵细胞团的正常形成具有显著的影响。 - The growth speed of clone is different in various inoculating density . the experiment result indicates : lower is inoculating density , faster the speed of growth is
在不同接种密度下,克隆的生长速度不同,实验结果表明:分瓶数量越多,克隆总重量增长越多。 - This article mainly discusses the large - scaled cultural conditions of clone in gametophyte laminaria . it studies the proper temperature , light time , light intensity , the consumption of nitrogen and phosphorus , the cultural state and inoculating density on the growth of gametophytic clone , the experiment of large - scaled culture had been done under comparatively proper conditions , in the same time , we studied the growth rule of gametophytic clone in the cultural process
本文主要探讨了海带配子体克隆的大规模培养条件,对海带配子体克隆生长的适宜温度、光照时间、光照强度、营养盐消耗、培养状态以及接种密度进行了研究,并在相对适宜条件下进行了大规模培养实验,同时,对海带配子体克隆在培养过程中的生长规律进行了研究。 - Primary culture of rat preadipocyte were prepared from the epididymal , inguinal and perirenal the fat pads of male normal , healthy , 15 - 20 days sprague - dawley rats . the preadipocyte grew better under the condition of 37 , 95 % humidity , 5 % co2 , ph 7 . 0 - 7 . 2 , centrifuged at 1000r / min , m199medium , and 10 % fetal bo vine serum , seeded at a density of 4 l04 , 5 l04 , / cm2 . oil red o staining was the special method to distinguish adipocyte from other cells , gimsa and he could determine the stage of the adiopcyte differentiation through the number of lipid drop , size and the position of the nucleolus of the staining fat cell
经过多次实验,确定本实验室大鼠前体脂肪细胞的最佳培养条件是:温度为37 ,湿度为95 , co _ 2浓度为5 , ph值为7 . 0 7 . 2 ,离心力为1000r / min ,培养基为m _ ( 199 )培养基,胎牛血清浓度为10 ,合适细胞接种密度为4 10 ~ 4 、 5 10 ~ 4个/ cm ~ 2 ,染色结果表明:油红o染色是鉴定脂肪细胞的特异方法, gimsa和he染色可根据不同区域染色程度、着色差别判断细胞核的位置及脂滴大小、多少,观察大鼠前体脂肪细胞分化过程中的形态变化,进而确定脂肪细胞的分化阶段。