扩增引物 meaning in Chinese
amplification primer
amplimer
Examples
- Two specific primers were designed and synthesized based on the conservative sequence contrasted to drosophila and other species mt gene by computer retrieving , which were named primerl and primer2 , the sequence of primer1 was : 5 " caa gtg aat cat ctc agt gc3 " , and that of primer2was : 5 " tgt aga gag aca aga tgc ag 3 " . rt - pcr technique was adoped to synthesizing the first - band of cdna , the second - band of cdna and expanding dna directly only by one step
根据已发表的果蝇以及其它生物的mt基因的保守序列，通过计算机同源检索比较，设计两个扩增引物，命名为引物1和引物2 ，引物1的序列是： 5 ’ caagtgaatcatctcagtgc3 ’ ，引物2的序列是： 5 ’ tgtagagagacaagatgcag3 ’ 。 - The methods of both amplification and dna sequencing with new primers were established . polymorphisms of mtdna control region in 5 ethnic groups in china were analyzed using the established methods to provide a basic database . molecular evolution and phylogenetic issues were discussed based on the mtdna database to understand genetic relationship of different ethnic groups in china
目的为了提高线粒体dna这一遗传标记的法医学鉴别能力，设计适合法医学检材的、覆盖mtdna控制区的扩增引物，建立新的扩增与测序方法；应用所建立的方法调查多民族mtdna控制区多态性，为涉及不同民族的mtdna序列分析提供群体遗传学基础；应用获得的数据进行分子进化分析，探讨各民族间的亲缘关系及同一民族内的分子进化问题。