性质粒 meaning in Chinese
cosmid
sex plasmid
virulence plasmid
Examples
- So some methods suitable to large plasmid extraction , including lysis by sds and a method from a literature , were used to try to extract the large plasmid from the strain cell . the lysis reactions in these two methods are gentle , so the large plasmid cannot be injured in the lysis process , opposite to lysis by alkali . it would be helpful to keep the integrality of the large plasmid during the extraction
因此我们采用了适合于大质粒提取的sds法，和文献中应用于硝基苯降解性质粒的提取方法，来尝试对菌株细胞进行质粒提取，这两种方法裂解反应温和，不会像碱裂解法那样，在裂解过程中损坏质粒，可以实现质粒提取的完整性。 - Sequence analysis shows that they share 98 . 75 % similarity at the dna , and 98 . 67 % at the protein level . ns2 gene was cloned into the multiple cloning sites of prokaryotic expression vector pgex - 6p - l . the recombinant plasmid pgex - 6p - ns2 was constructed and transformed to the competent cell bl21 ( de3 ) plyss , positive bacterium strain was induced by iptg
将ns2基因插入到原核表达性质粒pgex - 6p - 1的ecor 、 bamh多克隆位点之间，将重组原核表达质粒pgex - 6p - ns2转化到bl21 ( de3 ) plyss感受态细胞中，获得了表达ns2基因的阳性亚克隆重组子，在含amp的lb液体培养基中培养，经iptg诱导表达，用sds - page分析表达产物。 - The nitrobenzene degradative plasmid in pseudomonas xn - 1 is discussed in this paper , based on the characterization of nitrobenzene degradation by strain pseudomonas xn - 1 , which attempts to do some prepared work for the research in molecule level of nitrobenzene degradation by this strain
本论文在对菌株pseudomonasxn - 1降解硝基苯的特性及降解条件优化的基础上，研究了菌株细胞内所含有的降解性质粒，希望能为今后对这株菌在分子水平上降解硝基苯的特性研究打下一些基础。