尿囊液 meaning in Chinese
allantoic fluid
Examples
- 12 - day - old spf chicken embryo was inoculated with various concentration of aiv ( h5 subtype ) . then allantoidal fluid was collected and virus titer was measured
将禽流感病毒( h5n1 )接种12日龄spf鸡胚，所得尿囊液浓缩后，一步法提取病毒rna 。 - Two ndv strains , lasota and f48e9 , were propagated in 9 - day - old embryonated specific - pathogen - free ( spf ) eggs respectively for 36 hours at 37 , then these chicken embryos were placed at 4 for 12h before the allantoic fluid was gained
首先，将ndvlasota株和f48e9株分别接种于9日龄spf鸡胚，于37孵化36h ，置4 12h后收获鸡胚尿囊液。 - The rna genome of virus used for rt - pcr can be isolated not only from the infected chicken embryonated fluids , but also directly from tissue homogenate of field samples . the detection results of 67 field samples were identical with that of the conventional diagnosis
本方法只需一个rt - pcr反应，整个过程在8小时内完成，既可取感染鸡胚尿囊液检测， 2002年）丫h人《 ‘ wif寸它义：也可从病料直接检测。 - Materials and methods : fecal , cloacal and tracheal swabs from different types of poultry were collected in 6 live - bird retail markets once a week . they were inoculated into 9 - 11 days embryonated chicken eggs and incubated in 35 " c for 72 hours . hi and nl assays were performed to detect the subtype of viruses if ha test were positive ( ad ^ 16 ) and contamination test were negative
材料和方法：每星期收集一次标本，收集的标本常规处理， 9 - 11日龄鸡胚尿囊腔接种， 35培养72小时，收取尿囊液，血凝阳性( ad 16 )且细菌培养阴性者- 70保存并进一步做hi ， ni实验鉴定亚型。 - The research consist of four parts . the first part is multiplication , purification and electron microscope examination of the avian encephalomyelitis virus . a 1 : 5 dilution of isolate - nh937 of aev and control group of pbs were inoculated to susceptible 6 - day - old chickens embryos . respectively . after incubation for 10 days , the urinay vesicle liquid was collected . making a comparison the size of the chickens embryos between the test group and the control group , the results showed that the size of the control group is bigger than that of the test group . purified virions were examined under the electron microscope , the result revealed that there are a lot of virions and the aev - nh937 was multiplicated in embryos . the second part was seguence analysis of the genome of the aev - nh937 . nine pairs of primers were designed according to published calnek vaccine strain of aev
本研究共分四个部分：第一部分为aev的增殖，纯化和电镜观察，用1 : 5倍稀释的aev - nh937株和阴性对照pbs分别经卵黄囊接种于6dspf鸡胚，继续孵化10d后，收集尿囊液。比较接种组和健康对照组鸡胚的大小，结果显示，健康对照组鸡胚明显大于接种组。分离、提纯aev ，把纯化的病毒在电镜下观察，证明确有大量aev病毒粒子存在，说明aev在鸡胚中成功扩增；第二部分是aev - nh937基因组的序列测定工作。