小牛血清 meaning in Chinese
calf serum
cs
Examples
- Soleosery eye oint
小牛血清眼膏 - The cell - matrix complex was incubated with dmem and 10 % bovine serum under condition of 37 , 5 % co2 - the medium was changed daily . ( 3 ) cell - matrix complex paraffin section was made after 7 days incubation
将细胞悬液接种于戊二醛交联的胶原一壳聚糖多孔膜，加dmem 10小牛血清培养基后放置37 ’ c ， 5 co 。 - Not only the dp , but also the type and the original source of cellulose pulp , affect the permeation properties of the membranes but has almost no effect on the rejection of bovine serum albumin ( bsa ) solution
浆粕种类对纤维素膜的水通量影响较大，但对截留率(小牛血清蛋白溶液)影响不大。浆粕的聚合度、来源、组成对膜性能都有很大影响。 - The primary results showed : using m199 as diluents containing 20 % bovine serum , it is better to freeze the cells slowly freezing at fist then increase freezing speed ( for example , from 0 to - 6 freezing speed is about - 0 . 05 a minute , from - 6 to - 40 , freezing speed is about - 0 . 5 a minute ) , studies on effect of various concentration of dmso demonstrate that about 12 . 5 % dmso gave the highest post - thaw percentage of viable cells . the concentration of bovine serum had no different effect on the percentage of the viable embryo cells of misgurnus auguillicaudatus . the embryo cells derived 6 from the later stage of blastula offish is more resistant to the cryogen than the cells of early stage of blastula . the cells preserved in liquid nitrogen at - 196 were thawed and cultivated , a few cells were found adhere to the surface of culture vessel when the percentage of viable cell was more than 30 % . the cells in only two culture vessels were found to proliferated and gave rise to many small morphologically undifferentiated cells
研究初步表明:以细胞培养液m199 (含2既的小牛血清，常规量双抗)为冻存稀释液对泥鳅胚胎细胞冷冻保存宜采取先慢后快的方式(例如，从0一一6 ，冻存速度为一0 . 05 / min ，再以一0 . 5 / min的速度从一6一一40 ) ; dmso的保护效应浓度为12 . 506左右;小牛血清的浓度对泥鳅胚胎细胞的成活率影响不明显;囊胚晚期细胞抗冻性比中早期强;通过对不同批次的冻存细胞解冻培养，解冻后成活率为30 %以上细胞培养数天后均有少数细胞贴壁，但只发现两瓶培养细胞有明显增殖现象产生许多未分化的小细胞。 - Using m199 containing 20 % calf bovine serum and 11 % dmso as the diluent and by the methods using in cryopreservation of embryo cells of misgurnus auguillicaudatus , two groups of cells derived from blastula of grass carp were preserved in liquid nitrogen at - 196 . after 6 days cryopreservation , one group of cells were thrawed and the percentage of viable cell was about 72 % ; the other , cryopreserved for 13 days , was 52
以细胞培养液m199 (含20 %的小牛血清)为稀释液， dmso的浓度为11 % ;与泥鳅胚胎细胞冷冻保存方法一样，采取先慢后快的方式，冷冻保存两组草鱼囊胚晚期细胞于一1 %的液氮中。第一组冷冻保存6天后解冻，成活率为72 % ，第二组冷冻保存13天后解冻，成活率为520 / 0 。