寡聚脱氧核苷酸 meaning in Chinese
cpg odn
oligodeoxynucleotide
Examples
- And the two pah ' s of pcr primers that bind to the adapter and the sequence of f fragment close by tn5 respectively were also designed . the genomic dna of b8 was isolated , digested with bamh i , and ligated to the adapter . using the two pairs of the primers , two rounds of pcr were performed hi turn and a fragment of 239bp was amplified successfully . lt was proved by cloning and sequencing that 18bp of the fragment is the sequence opposite to f fragment on the left of tn5 insertion site in b8f , the other is part of the 728 bp of f fragment . this result makes it possible to continue to carry out chromosome walking , to clone and sequence the whole genes of b fragment and f fragment , and to reveal the antagonistic molecular mechanism of b8
试验研究设计并合成了由40和44个碱基的寡聚脱氧核苷酸组成的染色体爬行接头，在接头序列和测定的f片段近tn5的序列上，设计了2对染色体爬行用的pcr引物，从b8菌株中提取基因组dna ， bamhi酶切，与染色体爬行接头连接，依次用2对引物进行pcr ，扩增出239bp产物，经克隆、测序，发现其中18bp为扩增的相应于f片段在b8f菌株tn5插入位点对面的序列，其余则为f片段728bp序列的一部分，为进一步进行染色体爬行，克隆和测定整个b和f基因，揭示阳菌株的拮抗分子机制提供了技术资料贮备。 - The results showed mn and ni complexes possibly bind to dna by the mode of interaction , whereas zn complex possibly bind to dna by the modes of interaction and electrostatic binding . 5 . in addition , we conjugated cleavage system with recognize system and analyzed joint products by hplc , which provide experimental basic for design of dual effects cleavage
此外，本文还选用咖啡酸纯品来突破切割体系与识别体系(用氨基臂修饰的寡聚脱氧核苷酸)的连接，并用高效液相色谱法分析其偶联产物，为今后设计并合成一种具有特异识别和高效切割双重功能的人工核酸酶提供了实验基础。