寡聚核苷酸 meaning in Chinese
n-odn
odns
oligonucleotide
Examples
- Rapd ( random amplified polymorphic dna ) , which bases on the polymerase chain reaction ( pcr ) , is by far one of the most commonly molecular techniques to uncover dna sequence polymorphisms . the basic priciple of this technique is that an arbitrary primer ( usually lobp oligonudetide ) is used to amplify random segments of dna , and a small number of fragments will be amplified when the primer anneals on each strand over a length range . if sequence variation is present at the priming site , then a fragment may not be amplied , so the dna polymorphic can be detected
Rapd (随机扩增多态性dna )技术是二十世纪90年代发展起来的一项dna分子多态性检测技术，它建立于聚合酶链式反应( pcr )技术基础之上，利用随机合成的寡聚核苷酸序列为引物(一般为10个bp ) ，分别与dna的两条单链结合，在dna聚合酶的作用下，对基因组的特定区域进行pcr扩增，其电泳结果为不同大小和数目的dna谱带即rapd图谱，可反映基因组相应区域的dna多态性。