定点诱变 meaning in Chinese

site-directed mutagenesis

After site - mutation using pcr was introduced into cryld sd sequence ( gggga - ggagg ) , cryld - lacz expressed higher 1 . 0 - 1 . 6 times than before mutation , suggesting that ggagg might be most effective sd sequence for bacillus thuringiensis , meanwhile , low translational initiation efficiency caused by improper sd sequence might lead to low expression of cryld in hd133
利用pcr定点诱变技术突变其sd序列gggga为ggagg后， cry1d - lacz融合基因的表达提高了1 . 0 - 1 . 6倍。表明ggagg同样是苏云金芽胞杆菌最有效的sd序列，同时也揭示了基因cry1d表达量低的原因之一是不合适的sd序列而导致较低的翻译起始效率。