孵化酶 meaning in Chinese
hatching enzyme
Examples
- From the above results it can be concluded that the dorsal - anterior area and the dorsal area are probably the main position of he secretion
孵出后仍残留的孵化酶有可能参与了卤虫幼体内残余卵黄颗粒的消化降解，此结论还需进一步验证。 - The hatching enzyme from brine shrimp , artemia shrimp , is a pivotal protease which help the encysted embryo escape from its hatching membrane when hatching
卤虫孵化酶( he )是由卤虫早期胚胎特异性分泌的、在孵化过程中起关键作用的一种蛋白酶。 - The he was strongly inhibited by sbti and apmsf , and very sensitive to pmsf , lbti , and tlck , while not sensitive to chymostatin , bestatin , leupeptin , tpck , pepstatin , nem and iam . all these results imply that brine shrimp he was most probably a trypsin - type serine protease . the he could be strongly inhibited by edta in a dose - dependent manner , and 50 mmol / l edta exhibited more than 56 . 5 % inhibition
对孵化酶纯化样品进行生化性质和酶性质分析发现，卤虫孵化酶的最适反应温度约为40 ，最适ph为8 . 5左右；该孵化酶对p - apmsf 、 sbti极为敏感，对pmsf 、 lbti和tlck也非常敏感，但对chymostatin 、 leupeptin 、 pepstatin 、 bestatin 、 tpck 、 nem和iam不敏感，表明该酶极可能是一种属于胰蛋白酶类型的丝氨酸蛋白酶。 - The distribution of the brine shrimp hgcs varies greatly from the species studied till now . one hour after hatching , neither the dorsal - anterior area nor the other dorsal area remained positive immunoreactivity signal . and 2 hours after hatching , there was no typical hgcs in the body of the brine shrimp and the remained hatching enzymes may participate in digesting the left vitellin in the nauplius
卤虫hgc最初出现至孵化前1h时均为全身性分布，从孵出到孵出后2h ，头卤虫孵化酶的生物化学性质及孵化腺细胞的免疫组织化学研究部的孵化酶颗粒已经减少，而变为非全身性分布，到孵出后sh ，孵化酶颗粒已基本消失殆尽。 - The brine shrimp he was prepared and purified by 67 % ammonium sulfate precipitation , deae - sepharose fast flow ion - exchange chromatography , and sephacryl column gel - filtration , and its biochemical and enzymological properties were identified in this study . it was found that the deduced molecular weight of he in sds - page is about 98 . 5 kda , and its proteolytic activity was optimized at ph of 7 . 5 - 8 . 5 and at temperature of 40 , respectively
我们利用67硫酸铵沉淀、 deae - sepharosefastflow阴离子交换柱层析和sephacryl凝胶过滤柱层析，并以酪蛋白为其蛋白酶水解活性的检测底物、以卵膜为其卵壳裂解活性的特异性底物，从卤虫胚胎孵化液中分离纯化出了卤虫的孵化酶分子，其在sds - page电泳中的分子量约为98 . 5kda 。