子片段 meaning in Chinese
sub segment
sub-segment
Examples
- Promoter - probe vector psupv4 was used to clone promoters of pseudomonas pseudoalcaligenes in escherichia coli . nine kanamycin resistant recombinants were obtained and designated as ppal - ppa9 . the ppa7 , which has the highest kanamycin resistance , was chose for further characterization
利用启动子探针型载体psupv4直接在大肠杆菌中克隆类产碱假单胞菌( pseudomonaspseudoalcaligenes )基因启动子片段，获得具有强启动子的重组子ppa7 。 - The promoter - probe vector phn117 in e . coli and phn127 in g " were further constructed by removing promoter while keeping sd sequence from phn115 . a teta / tetr bidirectional promoter fragment from pbr322 was respectively cloned into phn117 and phn127 and the resulted colonies were all fluorescent
并经插入pbr322上665bpteta tetr双向启动子片段后得到的转化子均发绿色荧光验证，实现了wtgfp在大肠杆菌和华癸中生根瘤菌中的组成型表达。 - Besides , vgb gene expression also increased the chitinase secretion . in order to make the vitreoscilla hemoglobin gene express in bacillus subtilis , an inducible promoter ( levansucrase ) was cloned from b . subtilis wb600 and ligated with a promoter - less vgb gene . the resulted gene is called sacvgb and was demonstrated to express in e , coli by sds - page and carbon monoxide binding assay
由于vgb基因启动子不能在枯草杆菌中启动表达，因此，根据已发表的果聚糖蔗糖酶基因( sacb )序列设计引物，从枯草杆菌wb600总dna中扩增出该基因的启动子片段，然后将其与vgb基因编码区及终止子序列相连，成功地组建了sacvgb融合基因。 - Homologious analysis showed that it belongs to a small - member gene family . to study the expression pattern of st901 gene in detail , the six promoter fragments were amplified by pcr and fused to the gus report gene . the plant expression constructs were stably introduced into tobacco by agrobacterium - media transformation
为进一步确认st901基因的表达模式，利用pcr技术及启动子序列内部的酶切位点，克隆得到六个不同长度的启动子片段( 1368bp 、 680bp 、 335bp 、 319bp 、 300bp和288bp ) ，分别与gus基因连接构建植物表达载体，农杆菌转化烟草(马铃薯) 。 - Genomic dna was extracted from light foliage by sds method . two fragments ( 1 ~ 378 , 378bp ; 73 ~ 378 , 306bp ) of ref promoter were amplified by pcr using different specific primers , and then sequenced . compared with the reported sequence in genbank , similarity of dna sequence was more than 98 % , so right sequences were cloned and modified
本研究通过sds法从橡胶树幼嫩叶片中提取基因组dna ，根据genbank报道的序列设计引物，通过pcr的方法对ref启动子378bp的序列进行缺失改造，克隆到了分别长306bp 、 378bp的ref启动子片段，与genbank报道序列相比较同源性高于98 。