多角体蛋白 meaning in Chinese
polyhedrin
Examples
- In order to enhance it ' s quantity of expression in bombyx mori cells and larvae of silkworms , we co - express bmnpv and hgm - csf in bombyx mori cells and larvae of silkworms , and investigate it ' s outcome
因此，本次实验中，我们将家蚕杆状病毒多角体蛋白与gm - csf融合表达，并研究其对gm - csf表达量的影响。 - A 12 bp sequence of the 5 " end from the polyhedrin protein gene of bmnpv was ligated to the 5 ' end of hbsag ( pres2 + s ) protein coding sequence by pcr . the fusion product coding for hbv surface antigen medium sized ( hbmp ) with 4 - additional aa of bmnpv polyhedrin protein was obtained
本研究通过pcr突变的方法，在hbsag ( pres2 + s )前s2序列的5 ’端融合了bmnpv多角体蛋白基因5 ’端的12个碱基，获得了融合乙肝表面抗原中蛋白基因( hbmp ) 。 - Hegf gene with his - tag at the end , which was derived from pet22 - egf , was in - frame fused to the carboxy - terminal of polyhedrin ( ph ) gene , which included the amino - terminal 116aa coding region . the polyhedrin - egf fusion gene ( named ph - egf ) was then cut out with ecorv and ecori , and was cloned between ecorv and ecori sites of pbacpak . 8 ( the result plasmid was named pbacph - egf and the ph - egf fusing gene was right under the control of ph promoter ) . the pbacph - egf structure was verified with restriction enzymes digestion and pcr
从pet22 - egf质粒中分离出末端带his - tag的egf基因，对位融合于多角体蛋白n端116个氨基酸基因序列的下游(命名为ph - egf ) ，并在两段基因间设计了凝血酶xa蛋白酶切位点，经过酶切、测序等鉴定正确后，克隆至pbacpak8中，使ph - egf融合基因置于多角体蛋白( polyhedrin ， ph )基因启动子控制之下，构建成重组转移载体pbacph - egf 。 - Fusion gene by pcr was inserted into bombyx mori baculovirus transfer vector pbacpak . 8 and contransfected with lineared dna of bm - bacpak6 virus into bmn cells . the homologous recombination occurred inside the cells , and the recombinant virus bacpak - 6aa - hgm - csf was expressed , as identified by pcr and southern hybridization . the bmn cells and the fifth instars were infected by the recombinant virus bacpak - 6aa - hgm - csf
本研究首先通过pcr将家蚕杆状病毒多角体蛋白起始密码子后的18个碱基引入到hgm - csf基因的5 ’端之前，然后将融合基因重组与家蚕杆状病毒转移载体pbacpak8中，获得重组转移载体pbacpak8 - 6aa - hgm - csf ，并与线性化bm - bacpak6dna共转染家蚕细胞株，获得重组病毒bacpak - 6aa - hgm - csf 。