外切核酸酶 meaning in Chinese
exonucleases, bacterial
Examples
- The nucleotide ( nt ) sequence of the insert in phz1754 is 2299bps in size . computer assisted analysis of the sequence revealed an open reading frame ( orf ) with a g + c content of 70 . 3 % that would encode a protein of 552 amino acids ( aa ) . the nt seque nce comparision revealed that the orf in the sequenced region exhibits 85 % dna sequence homology with the cholesterol oxidase gene choa of streptomyces sp
对phz1754进行外切核酸酶( exonuclease , exo )顺序缺失,获得单向长度渐减重叠的系列突变体,核苷酸序列测定显示出该ecor - sal片段的精确大小为2299bps , frameplot程序分析揭示出该区域一个完整的开放阅读框( orf )的存在,其大小为1656bps , g + c含量为70 . 3 ,编码552个氨基酸,利用blastsearch程序将orf的核苷酸序列及推导的氨基酸序列与因特网上基因及蛋白质数据库进行综合比较,发现无论在核苷酸水平还是在蛋白水平上,该orf均与胆固醇氧化酶表现出同源性,而且与链霉菌胆固醇氧化酶同源性最高,说明该orf编码胆固醇氧化酶基因。