基因池 meaning in Chinese

gene pool

Cercariae were collected , cultured in vitro and transformed to schistosomula in the rpmi 1640 medium with rabbit serum . the schistosomula were cultured in conditional media up to 96 hours . the number of schistosomula was counted and the death rate was calculated
将条件培养基与童虫共培养， 96h内连续观察计数，计算童虫死亡率，与阴性对照比较，童虫死亡率最高的基因池进入下一轮筛选。
These results are very important for us to further understand the genetic background , biological characteristics , evolutionary rule and the anti - schistosomajaponicum mechanism of microtus fortis at the molecular levels . the specific base changes of the dna fragme nt between the two subspecies are probably correlative with the animal immigration , survival conditions , and species evolution . the cdna library of microtus fortis bone marrow cells was transferred in situ to nylon membrane , which was divided into eight equals ( ga ~ - gh )
利用已经建立的东方田鼠骨髓细胞质粒cdna文库，将cdna文库转化菌落印迹至尼龙膜，将膜均分成8份( ga gh ) ，制备基因池，分别培养、提取基因池质粒dna ，通过lipofect - 2000脂质体转染技术，将基因池质粒dna导入hek293细胞， 48h后收集转染细胞上清液，即条件培养基。