地高辛标记探针 meaning in Chinese
digoxin labelled probe
digprobe
Examples
- Through in situ hybridization and colony pcr , a positive recombinant plasmid was isolated from the genomic library and sequenced . the inserted dna fragment was about 4 . 3 kb
将pcr扩增片段用地高辛标记探针，利用原位杂交和菌落pcr从基因文库中获得含有阳性信号的重组质粒。 - Selected one of the 14 strains - s93 , s93 dna was digested partially with sau3a i and 2 ~ 3kb fragments were collected and inserted into puc 18 , then transformed into dh5 a . filtering the clone with hybridization in situ , a 1 kb frament clone has been cloned
使用sau3ai对基因组dna进行不完全酶切，回收2 3kb片段，与puc18质粒连接转化大肠杆菌，利用地高辛标记探针，使用菌落原位杂交筛选转化子；筛选到包含有约1kb外源片段的转化子。