可溶性抗原 meaning in Chinese
soluble antigens antigens
Examples
- After the recombinant plasmid pcdna3 . 1 / ts87 was identified by digestion of hindlll and bamh i , it transformed into cos7 by lipofectamine . expression product was identified by immunohistochemical method , sds - page and western - blot . the immunocytochemistry result has shown that specific brown - staining grains were found in the cytoplasm of cells transformed by recombinant plasmid versus not seen in cells transformed by pcdna3 . 1 or normal cells ; the sds - page result has revealed that a band about 3 8kb was found in cell lysis transformed by recombinant plasmid versus not in cells transformed by pcdnas . l or normal cells ; the western - blot result has showed that only the band about 38kd was recognized by sera from rabbit infected by t . s artificially and sera from rabbit immunized with soluble antigen of t . s and with protein expressed by ts87 gene and by a monoclonal antibody of t . s
通过细胞的免疫组化，细胞裂解物的sds - page电泳， westem - blot分析检测目的基因的表达情况。免疫组化结果显示：重组质粒转染的细胞质中有棕褐色颗粒，而空载体转染细胞及正常细胞无此现象；细胞裂解物sds - page电泳结果显示：只有重组质粒转染的细胞在约38kd处有明显的蛋白带，这与理论计算的ts87基因表达蛋白的分子量为38kd基本一致； western - blot分析结果显示：约38kd的蛋白带能够分别被旋毛虫感染兔血清，成虫虫体可溶性抗原免疫兔血清， ts87基因原核表达蛋白免疫兔血清( ts87血清)以及一株具保护性的旋毛虫单抗特异识别。