前病毒 meaning in Chinese
provirus，pv
Examples
- The env protein deduced from env gene encodes the hydrophilic surface protein ( su ) and the hydrophobic transmembrane domain ( tm ) that determine the specific interaction between virus particles and cell surface receptors during retroviral entry . the su of retroviruses is a highly variable genetic element , containing receptor binding sites and major antigenic determinants . exjsrv - specific dna probes were derived . by using these dna probes in tissue hybridization . we successfully identified jsrv mrna expression and proviruses dna in sheep lung tissues infected with jsrv and control group has no postive signals , validating the use of exogenous virus - specific dna probes in the analysis of oncogenic proviral integration sites and identification of integrated exogenous proviral sequences
用地高辛随机引物法标记exjsrv特异的env片段，制备探针，原位杂交检测spa肺组织中的rna及前病毒dna ，结果表明spa患羊肺组织内有jsrvenv基因mrna的表达，同时也检测到了前病毒dna ，而相应的阴性对照却无阳性信号，证实外源性病毒特异的dna探针在致瘤性前病毒的整合位点和整合的外源性前病毒的检测中具有可信度。 - The proviral genome cdna clone of snv strain was digested , and the fragments was cloned into the puc18 vector , sequenced , finally we got the sequence of snv genome . the two pairs of primers were designed and synthesized according to the snv strain env sequence . the env genes of the rev isolates isolated from china were amplified by polymerase chain reaction ( pcr )
根据网状内皮增生症病毒snv株env基因的序列，设计并且合成了两对引物，利用该引物，以中国地方分离株sd9901 、 ha9901前病毒基因组cdna为模板，通过pcr技术，成功的从国内分离得两株rev毒株中扩增出env基因，并将之克隆进pucm - t载体中测序。 - The eiav - pok8 . 2 - his was transfected into the donkey leukocyte culture . following an incubation period , reverse transcriptase activity was detected in cell culture supernatants . cytopathic effect was observed by no . six passages post - infection in donkey leukocyte infected by the virus derived from eiav molecular clones eiav - pok8 . 2 - his
提取前病毒dna ， pcr扩增p1p4片段，亚克隆至pmd18 - t载体，测序证明前病毒dna含有六个组氨酸，从而在体外获得了感染性克隆病毒株。 - Using the monoclonal antibody to rev and the anti - sera against the rev env gp90 - gst fusion protein . the molecular cloned virus was detected by ifa . we also amplified the gp90 from the cells infected with the molecular cloned virus by polymerase chain reaction . all these results indicated the recombinant plasmid containing the total rev genome cdna is infectious
对snv株前病毒全基因组cdna克隆进行酶切，将酶切产物分别克隆进puc18中，分别将各个亚克隆进行测序，按照酶切位点和已知的部分序列以及rev物理图谱将测得的序列进行拼接，完成了rev全基因组序列。 - The donkey - adapted strain of eiav ( dv116 ) , parental strain of eiav - dla , is a highly virulent isolate which was developed by sequential passaging a virulent eiav strain in donkeys in 1970s . the donkeys inoculated with fatal doses of eiav d strain can always be killed within in the first acute disease period . in this study , we constructed a full - length provirus dna clone of dv116 by pcr
本实验中将eiav驴强毒dv体外感染驴白细胞培养物，一定时间后收获病毒(本文中简称dv116 ) ，提取eiav前病毒dna ，以pcr法分别扩增并克隆了包含全长基因的三段前病毒dna片段，以双脱氧法测定了dv116病毒全基因序列共8236个核苷酸。