分级沉淀 meaning in Chinese
fractioanl precipitation
fractional precipitation
fractionalprecipitation
fractionated precipitation
fractionation precipitation
Examples
- 3 . the extracellular phb depolymerase was purified from 09 by using hydrophobic column chromatography and gel filtration technique in sephadex g - 100 . the specific activity of the purified enzyme was increased by 37 . 9 folds over crude extract , and the recovery yield was 8 . 9 %
以粗酶液为起始，经硫酸铵分级沉淀、 sephadexg - 100凝胶过滤后，分离纯化了该酶，纯化倍数约为37 . 9 ，酶活力回收率8 . 9 。 - Medium experiments were arranged under uniform design , and then an optimum medium was got accordingly . the culture liquid was centrifugalized at 3 , 500r / min for 30min , then ammonium sulfate was added into the supernatant to a final concentration of 30 % to precipitate the others
通过硫酸铵分级沉淀、 deaesephadexa - 50阴离子交换凝胶层析和sephadexg - 75凝胶柱层析对发酵液进行分离和纯化，并得到电泳纯的酶。 - The crude cellulases from liquid fermentation of b - 6 and ass . 3711 were isolated and purified by ( nh4 ) so4 precipitation , sephadex g - 100 and deae - sepharose cl 6b column chromatography . the cmcase components were purified and some of their physical and chemical properties were studied
本文将液体发酵的酶液经硫酸铵分级沉淀、柱层析后得电泳纯cmcase组分，并对as3 . 3711和b - 6来源的cmcase酶解动力学和理化性质作了比较研究。 - - acetolactate decarboxylase is purifed from cell extract by 50 % - 80 % ammonium sulfate - fractionation , 50 , 2min heat treatment and deae - sepharose fast flow column chromatography , which we study the different ph and different buffer of deae - sepharose fast flow column chromatography and conclude ph 6
对其酶学性质进行了研究。 -乙酰乳酸脱羧酶经50 80硫酸铵分级沉淀、 50 ， 2min热处理、 deae - sepharosefastflow离子交换柱层析方法分离纯化。 - Crotalaria mucronata lectin ( cml ) was purified from seeds of crotalaria mucronata by extraction , fraction with ( nhi ) 2864 , hog gastric mucin - sepharose 4b affinity chromatography and followed by gel filtration of sephacryl s - 200 hr . cml agglutinated type a human red blood cells specially . the purified cml gave one band pel e ' ectronhoresis and on sds nolvacrvlamide gel electrophoresis
野花生豆( crotalariamucronata )经磨粉、浸取、硫酸铵分级沉淀、猪胃粘蛋白- sepharose4b亲和层析、 sephacryls - 200hr分子筛层析可得到一表观分子量为103kd且对a型血红细胞专一凝集的野花生豆凝集素( crotalariamucronatalectin ， cml ) 。