几一 meaning in Chinese
kiichi
Examples
- Being one of the three key knots guaranteeing safety and reliability of pile foundation , pile foundation inspection occupies an irreplaceable position in engineering
摘要桩基础检测作为确保桩基础安全与可靠必不可少的三个重要环节几一,在工程中有其不可替代的地位。 - These results demonstrate that nps in m3m4 loop is the b cell epitope recognized by mab363 , which may be important for developing a practical immunization strategy against excitotoxic brain injury
、 ifn一y 、 il一4 、几一6明显增加,与对照组之间有显著性差异,说明小鼠机体内产生了细胞免疫和体液免疫反应。 - Here we managed to make cultured mice peritoneal macrophages be directly influenced by oligochitosan , and be stimulated by ifn - r before oligochitosan added , then measured the changes of gene transcription and translation level of both il - 1 and imf - a , respectively by methods of relatively quantitive rt - pcr and elisa . first , rt - pcr results showed that 18 hours was the most effective time and 40ug / ml was the most effective concentration of oligochitosan , then by the same method , confirm that 4hours is the most effective time and loou / ml is the most effective concentration of ifn - r stimulating . because ifn - r can enhance il - 1 and tnf - a gene expression of macrophages alone , so add ifn - r to microphages alone for 22 hours , then examined by rt - pcr , the results showed that il - 1 and tnf - a gene expression have no remarkable difference compared with the blank contrast group
此外,由于ifn y单独作用也可促进两种细胞因子基因表达,故在巨噬细胞中加入ifn y单独作用22h ,再经阿一pcr检测,发现加ifn y的实验组细胞的几一lp和tnf a基因转录水平与空白对照组相比较无显著性差异,可见,壳寡糖和ifn v对巨噬细胞il lp和tnf一口基因转录水平的影响在作用时间上无一致性,在壳寡糖作用最适时间时,仅受ifn y刺激的巨噬细胞il lp和tnf q基因转录己下降至刺激前水平,因此可以认为, ifn y的加入仅起到对巨噬细胞预刺激使之处于敏感状态的作用,有利于增强壳寡糖对巨噬细胞的作用。 - 2 specific epitope analysis in m3m4 loop target to determine the b cell epitope of a monoclonal antibody against the m3 - m4 loop of nmdar1 , a random phage displayed dodecapeptide library was screened with the monoclonal antibody mab363 against the m3m4 loop of nmdar1 . after four rounds of biopanning , the peptide sequences of positive phage clones were determined and analyzed by dna sequencing
流式细胞仪测定脾细胞cd4 + 、 cds寸淋巴细胞亚群,间接elisa测定血清thl细胞因子几一2 、 tn下一a和n一y , th一2细胞因子几一4 、 il巧和几一6的水平,同时检测特异性抗体产生情况。