凝胶层析 meaning in Chinese
gel chromatography
Examples
- An active metabolite was obtained by purification with precipitated by ethanol , sephadex g - 25 gel , deae - cellulose ion exchange resin and silica gel column chromatography
经乙醇( 95 )沉淀、 sephadexg - 25凝胶层析、 deae -纤维素离子交换层析和硅胶层析纯化，得到抑制黑曲霉生长的单一组分。 - In this thesis , we have done some research on the characterization of cu , zn - sod from hog blood by polyacrylamide gel electrophoresis , electric focusing electrophoresis and dealing with some different reagents
本文采用聚丙烯酰胺凝胶电泳，巯基已醇- sds -聚丙烯酰胺凝胶电泳，等电聚焦电泳以及各种凝胶层析的方法对猪血铜锌超氧化物歧化酶进行了研究。 - Medium experiments were arranged under uniform design , and then an optimum medium was got accordingly . the culture liquid was centrifugalized at 3 , 500r / min for 30min , then ammonium sulfate was added into the supernatant to a final concentration of 30 % to precipitate the others
通过硫酸铵分级沉淀、 deaesephadexa - 50阴离子交换凝胶层析和sephadexg - 75凝胶柱层析对发酵液进行分离和纯化，并得到电泳纯的酶。 - Firstly , the major ampullate glands were got from araneus ventricousus . total rna and mrna of ampullate glands were isolated and purified . double strands cdnas were synthesized in the assistance of amv - rt and e . coli dna polymerase i etc . by reverse transcription and replacement synthesis
首先剥离大腹园蛛主壶腹腺，提取总rna和分离纯化mrna ，反转录合成cdna ，凝胶层析除去小于400bp片段，并在双链cdna两端引入ecor的衔接头，对其进行磷酸化处理。