兔化毒 meaning in Chinese

lapinized virus

It has made the strong basis for further studying mechanism of replication , virulence and determinant , attenuation , pathogenesis , functions of genetic products , specific diagnosis , cell and host tropism , development of dna vaccine and marker vaccine of csfv , and provided an excellent tool for molecular virology . main research contents include : based on published nucleotide sequences of csfv and by the help of computer analysis software , high conservative regions and single restriction enzyme sites of genome were selected . utilizing rt - pcr and nested - pcr techniques , 7 overlapping cdna fragments covering the full genome of csfv c - strain were successfully amplified
中国猪瘟兔化毒(脾淋毒)基因组cdna文库的构建、序列分析：根据已发表的猪瘟病毒( csfv )核苷酸序列，借助计算机软件分析，选择高保守区段和基因组中的单一限制性酶切位点，利用rt - pcr及nested - pcr和helf - nestedpcr技术，成功地扩增出了覆盖c -株全基因组的7个cdna重叠片段f1 f7 ，分别克隆到pmd - 18t或pgem - teasy载体进行测序后，拼接出了其核苷酸序列。