克隆序列 meaning in Chinese
cloned sequence
Examples
- Cdna cloning , sequence analysis , expression and protein structure modeling of hla - g1 from chinese
中国人hla - g1的cdna克隆序列分析表达和蛋白质空间结构预测 - Contrasting analysis with the genes sequence of bacillus subtilis 168 strain ' s bio operon , the sequence of bio operon of as 1 . 1094 strain had 12 bases difference with that of 168 train , and 7 bases caused variation of amino acid
1094菌株与168菌株bioafdb基因序列完全一致， 9个差异碱基是由于pcr扩增导致的。枯草杆菌生物素操纵子基因4个亚克隆序列的测序结果证实， asi - Connecting the production of rt - pcr with the plasmid vector of puc19 and transforming the vector into ecoli bacillus , the recombinational bodies of chil - 1 were produced . then they were examined by pcr amplifying , enzyme digesting and sequence analysis
将rt ? pcr产物与puc19载体相连，转化大肠杆菌，经pcr 、酶切分析及序列测定得知所克隆序列与报道序列同源性达99 %以上，证明所克隆基因为目的基因。 - Part ii screening of tnfa mimotopes from phage display peptide library : based on the results of screening tnfa binding - peptides , we have tried to use neutral tnfa mcab j1d9 as target to screen tnfa mimotopes from c7c phage display peptide library , which may be another form of antagonist for tnfa , and the mimotopes were identified by sandwich elisa . after 3 rounds of screening , we got 9 phage clones identified as positive clones which can bind with mcab j1d9 . we also identified the binding between mimotopes and tnf receptor by competitive elisa , and the results showed strongly binding . the amino acid sequence results shown three different sequences : c - rrpaqsg - c - nkhnrki - c and c - rgmsrki - c
在对噬菌体环七肽库进行三轮亲和性筛选后，随机挑选20个噬菌体克隆， elisa鉴定出9个阳性克隆，经dna测序推出三种氨基酸序列： c - rrpaqsg - c 、 c - nkhnrki - c和c - rgmsrki - c ，其中优势克隆序列为c - rrpaqsg - c ；鉴定结果显示阳性克隆能够与tnf受体结合，并且能够阻断tnf与受体的结合，提示筛选得到的环七肽克隆展示肽具有tnf的抗原性及与tnf受体结第一军医大学顾士学位论文合的特性，为tnfa表位模拟肽。