体内法 meaning in Chinese

intracorporal method

The objective of this study was whether the reconstituted embryo with the second polar body ( pb2 ) can develop . firstly , female mouse was superovulated with pmsg + hcg and keep in the same cage with male mouse , the pronuclear embryo was obtained at the different time after hcg injection with different motheds to investigate the existing time of pronuclear embryo . then pronuclear embryo was cultured with three different mediums to select the best to culture in vitro
母鼠在注射hcg后与公鼠同笼交配，并在注射hcg后不同时间(从18h到26h ) 、不同方法(体内法和体外法)获取胚胎，研究获取原核胚的最佳时间；在此基础上获得具有第二极体原核胚，然后对原核胚进行显微操作，去除雌原核并注入第二极体，探讨第二极体内遗传物质支持胚胎发育的潜力；同时利用dna特异性染料h33342对原核胚进行染色，显示并比较原核和第二极体内的dna遗传物质。