传代数 meaning in Chinese
passage number
Examples
- The quality of feeder layer is affected by a lot of factors , such as animal breed , culture medium , passages in vitro and experiment condition , etc . as to the production of feeder layer , there are a few reports about morphological and histologic change when of embryonic body fibroblast when culturing in vitro and cryopreservation , so kunming mouse were chosen as experimental animals and morphological and histologic changes were studied in course of its embryonic body culturing . we expect to offer theoretical foundation to our laboratory for setting up feeder layer storehouse . at the same time , the feasibility of myocardium tissue culturing with fibroblast layer altogether was studied so that established foundation for studied the biological characteristic of heart outside body
小鼠胚体成纤维细胞的培养是制备饲养层的重要途径，其制备、传代及冷冻保存均有不同的研究报道，饲养层的质量受许多因素的影响，如动物的品种、培养液、所传代数及实验条件等，关于饲养层制备过程中的胚体细胞培养、传代、冷冻后的细胞形态、组织学等方面的研究报道很少，故本实验以昆明小白鼠为实验动物，研究其胚体培养过程中细胞的形态学、组织学等方面的变化，以期为本实验室建立饲养层细胞库提供理论依据，同时探讨心肌细胞和成纤维细胞层共培养的可行性，以期为心脏生物学特性的体外研究奠定基础。