人血小板 meaning in Chinese
human blood platelets
Examples
- Thus , the present study was undertaken to explore the possibility of constructing thrombopoietin ( tpo ) gene targeting vector specially expressed in mammary gland
为此，本研究对人血小板生成素乳腺表达打靶载体的构建进行了研究。 - The gene targeting vector was planning to be constructed by using thrombopoietin gene ( tpo ) gene as purpose gene , as 1 - casein gene as targeting gene , the 5 " sequence of - casein gene as promotor
以绵羊-酪蛋白基因的5调控区为启动子，人血小板生成素基因为目的基因，奶牛s1 -酪蛋白基因为靶向基因。 - Then , 5 . 5kb thrombiotin gene was amplified with the same technique from the genome of a baby ' s blood , which included the begining part of intronl to the teminator . in addition , 6 . 0kb and 1 . 8kb homlogous arms were also amplified from a cow with high yield . the 6 . 0kb homologous arm contains the promotor , extron 1 , extron2 , extron3 and intron 1 , intron2 and part of the intron3 fragment , while the 1 . 8kb homologous right arms contains exon13 , exon14 and part of intron 13 , the whole intron14 and part intron 14 of asl - casein gene of bovine
通过长片段pcr从高产奶牛的基因组中获得了打靶所需的长、短同源臂序列，长度分别为6 . 0kb和1 . 8kb ，位于s1 -酪蛋白基因的5上游区到第三内含子和十二到十四内含子；从绵羊全血基因组克隆得到了绵羊的-酪蛋白基因启动子区到第二内含子区4 . 1kb的5调控序列；利用同对引物克隆得到了水牛的同基因序列；从广西当地一婴儿脐血基因组中通过获得了人血小板生成素基因，位于第1内含子到终止子后部分的序列，长达5 . 5kb 。 - Purpose 1 construction of prokaryotic high expression vector of human platelet factor 4 ( h pf4 ) 2 expression and purification of r h pf4 3 bioassay of r h pf4 methods according to the modulation character of eukaryotic protein expression in prokaryotic cells , we design a pair of particular primers , and construct a prokaryotic expression vector pbv220 - r hpf4 by dna polymerase chain reaction ( pcr ) and dna recombinant technic . the expression plasmid was identified with pcr and dna sequencing . pbv220 - r hpf4 was transformed into e . coli dh5a , bl21 ( de3 ) and induced by increasing the temperature to 42 . we identified the expression protein by sds - page and western - blotting
目的1人血小板因子4 ( hpf4 )原核高效表达克隆的构建2重组hpf4的表达及分离、纯化工艺研究3重组hpf4的特性研究方法根据原核细胞表达真核蛋白的基因表达调控特点，设计合成一对特异引物，在pt7 - 7 - rpf4表达质粒的基础上，应用聚合酶链式反应( pcr )对其cdna进行改造，通过dna重组技术构建成重组hpf4原核表达质粒pbv220 - rhpf4 ，用快速pcr检测法、 dna测序分析，鉴定重组hpf4表达质粒的正确性。