亲株 meaning in Chinese
parent strain
Examples
- The acetaldehyde dehydrogenase ( acdh ) and alcohol dehydrogenase ( adh ) of the adhe mutants and parent strain were assayed with a result that the acdh activities of mutants were less than 10 % of the parent strain and the adh activities of mutants are about 10 % of the parent strain
6个adhe ~ -突变株的乙醛脱氢酶活性均降低为亲株的10以下，乙醇脱氢酶活性降低为亲株的10左右； 4个ldha ~ -突变株的乳酸脱氢酶活性均降低为亲株的7以下。 - First , after investigation of two original strains " biological characteristics , we studied the main influence factors on protoplasts formation and regeneration in s . mycarofaciens and s . erythreus , and determined the best protoplasts formation and regeneration conditions of two original strains . the former shake - cultured in s " medium at 28 ? , 220r . min ~ ( - 1 ) for 24h , lysised by 3mg / ml lysozyme , keeping warm at 32 ? for 50 ~ 60min , regenerated on r _ ( 5 " ) medium , 28 ? for 5 ~ 6d . the latter used two - step culture , then used img / ml lysozyme keeping warm at 37 ? for ih ; the protoplasts were plated on r5 " regeneration medium at 28 ? for 5d
首先在对两亲株的生物学特性进行了鉴定后，考察了影响两亲株原生质体形成和再生的主要因素，确定了生米卡链霉菌和红霉素链霉菌原生质体形成及再生的最佳条件：前者用s培养基，在28 、 220r . min ~ ( - 1 )培养24h后，用3mg ml的溶菌酶在32恒温酶解50 60min ，得到的原生质体在干燥的r5培养基上28倒置培养5 6天，可得到再生率在20左右的再生菌落；后者采用二级菌丝培养，用1mg ml的溶菌酶在37恒温酶解1h左右，得到的原生质体也在干燥的r5培养基上28倒置培养5天，即可得到再生率在20左右的再生菌。 - Irradiation or heat treatment protoplasts fusion were developed . equal protoplast suspensions ( containing about 108 / ml protoplasts ) of each strain were mixed and washed by centrifugation , then the pellets were resuspended in peg4000 solution ( 35 % ) at 37 ? for 2min . after centrifugation and resuspension , protoplasts were diluted and plated on regeneration medium
其次，确定了原生质体融合的基本条件及灭活原生质体融合的条件：等量混合两亲株原生质体或用热灭活(或紫外灭活)的原生质体( 10 ~ 8个ml ) ，用35的peg4000在37处理2min后，稀释，涂布再生，可获得融合率在50左右的融合子。 - Two pta - mutants have been selected by using suicide substrate after the mini - tn5 transposon insertion mutagenesis of klebsiella pneumoniae m5al . when used in microaerobic fermentation , the amount of acetate produced by the mutants reduced to less than 50 % of the parent strain , and the yields improved whereas the 1 , 3 - propanediol titers and productivities decreased
以klebsiellapneumoniaem5al为出发菌株，用mini - tn5随机转座诱变结合自杀性底物筛选的方法得到了两株产乙酸途径pta基因缺失突变株xl - 6和xl - 11 ，应用到微氧法发酵中，突变株的乙酸产量为亲株的50以下，甘油转化率有所提高，但1 ， 3 -丙二醇浓度和生产强度有所下降。