乙酰鸟氨酸酶 meaning in Chinese
acetyl ornithinase
acetylornithinase
Examples
- Acetylornithine deacetylase is the key enzyme of producting l - methionine . we mainly do research work on the construction of acetylornithine deacetylase gene - engineering strain and characteristic of proteinase . in order to get high expression deacetylase strain , we obtain the gene by pcr arge gene . the product ( 2800bp ) was cloned into puc19 plasmid and confirmed with blue / white dot screening > restriction enzyme analysis and pcr . then taking the nucleotide sequencing compared with the sequence at blast of u . s . a . we constructed a high expression of gene - engineering strain - pxj 128 which containing the arge gene on the high expressing system of pxji18 with activity of acetylornithine deacetylase above 20000u / g
为了获得高效表达的脱乙酰鸟氨酸酶工程菌株，在工程菌技术改造及其固定化研究做了进一步的研究和探讨。我们采用基因工程技术，通过pcr技术扩增出了酰化酶关键酶基因?脱乙酰鸟氨酸酶基因arge ，将其克隆到puc19载体中，经酶切鉴定、 pcr鉴定筛选出重组阳性质粒，并测序鉴定，通过美国blast程序进行了基因数据库相似性比较分析。