乙内酰脲 meaning in Chinese
glycolycurea
glycolylurea
glycolyurea
hydantoin
Examples
- To improve the production and activity of the enzyme , the hydantoin hydrolase gene ( hyuh ) was cloned into e . coli m15 and bl21 ( de3 ) by using vector pqe60 and pt221 respectively . two expression plasmids were thus constructed
用pcr技术从质粒puc18 - 169中扩增出l -乙内酰脲水解酶基因( hyuh )和n -氨甲酰基氨基酸水解酶基因( hyuc ) ，分别在大肠杆菌和毕赤酵母中实现了活性表达。 - Though the expression level of bl21 ( de3 ) / pt221 - hyuh was lower than that of m15 / pqe60 - hyuh , the target protein of bl21 ( de3 ) / pt221 - hyuh was principally in soluble form while the objective protein of m15 / pqe60 - hyuh was principally in insoluble form . both of the products in the two strains showed biological activity , but the former is 2 times higher than the latter . the hyuc dna fragment was inserted into ppic3 . 5k plasmid to construct the ppic3 . 5k - hyuc recombinant plasmid which was then transduced into pichia pastoris gs115 cells after being linearized by bgl ii digestion
结果表明， sds - page分析在50kd处有一较强的表达带，融合有分子伴侣的重组菌株bl21 ( de3 ) pt221 - hyuh与非融合表达的重组菌株m15 pqe60 - hyuh相比，乙内酰脲水解酶的表达量低一些，但其表达蛋白主要以可溶性形式存在，而m15 pqe60 - hyuh中表达蛋白则主要以包含体形式存在，且前者的酶活性是后者的2倍多。 - Puc18 - 169 was a subcloned plasmid containing the whole operon sequence of l - hydantoinase from arthrobacter bt801 which can convert 5 - benzylhydantoin to l - phenylalanine . hydantoin hydrolase which is responsible for the ring opening of hydantoin is one of the components of hydantoin utility enzymes of arthrobacter bt801 . n - carbamoylase is a part of hydantoinase operon which can transform n - carbamoylamino acids into the corresponding amino acids
节杆菌( arthrobacter ) bt801是由军事医学科学院生物工程研究所保存的l -乙内酰脲酶产酶菌株， puc18 - 169是由本室构建的含有节杆菌bt801的l -乙内酰脲酶完整操纵子序列的亚克隆质粒。 - Hydantoinases which consist of hydantoin hydrolase , n - carbamoylase and sometimes hydantoin racemase can hydrolyze d , l - 5 - substituted hydantoin into corresponding optically active amino acids . therefore , the hydantoinases from micro - organisms are widely applied to the industrial production of natural and unnatural amino acids . microbial hydantoinases are considered as industrial enzymes from the origin of life
乙内酰脲酶是广泛存在于动物、植物和微生物的一类酶系，包括乙内酰脲水解酶( hydantoinhydrolase ， hyuh )和n -氨甲酰基氨基酸水解酶( n - carbamoylase ， hyuc ) ，有些情况下还包括乙内酰脲消旋酶( hydantoinracemase ， hyur ) 。 - The l - n - carbamoylase of arthrobacter bt801 , coded by the hyuc gene , is the rate - limiting and the only stereoselective enzyme . hydantoin hydrolase gene ( hyuh ) and n - carbamoylase gene ( hyu c ) were amplified from the plasmid of puc18 - 169 by pcr and , at the same time , activity expression was obtained in e . coli and pichia pastor is gs115 respectively
节杆菌bt801的乙内酰脲水解酶催化乙内酰脲类化合物水解开环， n -氨甲酰基氨基酸水解酶是该菌乙内酰脲酶系中惟一具有立体选择性的酶，也是整个反应体系的限速酶。