上柱 meaning in Chinese

upper prop

Cloning the gene into a pgex expression vector clone the hbrp gene using pcr , restriction digest the pgex - 5x - 1 vectors and pcr product with bamh i and xho i , recovery the pcr product and pgex - 5x - 1 vectors
纯化样品样品上柱， pbs洗柱后，用洗脱buf企r将蛋白洗脱下来。利用蛋白收集器，收集蛋白，浓缩结果1
Without ammonium sulphate fractionation and dialysis , the supernatant of crude extract was directly loaded on deae - sepharose cl 6b column equilibrated by phosphate buffer ( 50mmol / l , ph7 . 8 ) , and the enzyme fraction was not absorbed on the column but impurities were absorbed
粗酶液无需硫酸铵沉淀及透析，即可引入磷酸缓冲液( 50mmol l ， ph7 . 8 )预平衡的deae - sepharosecl6b柱，上柱后用平衡缓冲液洗至基线稳定。 afpga不被吸附而直接流出。
In order to separate the l - lactic acid and lactic acetate , the adsorption chromatography was utilized besides the extraction with ethyl ether . the operation conditions for resin a was initial 15 % lactic acid , ph 6 . 5 , temperature 30 , flow velocity 2 . 28 ml / min taking the condition of optimal enzymatic reaction , the amount of adsorption and natural hydrolyzation of lactic acetate into account
综合考虑酯酶的最适反应条件、树脂吸附量和乳酸乙酯的自发水解情况，确定酶解底物乳酸乙酯浓度为15 ，上柱条件为； ph值为6 . 5 ，温度为30 ，流速为2 . 28ml min 。