ypd meaning in Chinese
粉胨葡萄糖琼脂培养基
Examples
- Multicopy clones were screened from vary levels of g418 - ypd plate and cultured and induced with methanol to secrete interesting peptide , which was identified by sds - page and western blotting
培养多拷贝插入的酵母细胞，用甲醇诱导目的蛋白的分泌， sds - page和westernblotting鉴定表达蛋白。 - The transfermants with highest resistance to g418 ( 4 g / l in ypd ) were screened to study their expression level in 150 ml shaking flask . having been induced with methanol for 36 hours , the target enzyme could be examined in the supernatant by measuring amidolytic activity
首次将大连蛇岛赅蛇毒类凝血酶成熟基回克隆到表达载体ppicgk中，经电激转化至毕氏酵母菌株gs15中，再经甲醇诱导，在150ml摇瓶畔1获得细胞外分泌表达产物。 - Construction and expression of yeast engineering yaccine : s14 / gnsag " as transformed to yeast host straln x33 by means of electroporation after ppiczaa s , . aresag " as l ined by saci enzyme . the single fungus , as choose and dibble inocu1ating in we and am plate , the positive fungus was gro ' ing in rm but not in w , and was 6 inoculated in ypd which included zeocine 500ug / ml and 1000ug / ml . 5 transformers were ampl if ied by pcr , three is same with positive control
选取单个菌落分别点种到删平板和md平板，找出在回d上生长正常， w上生长缓慢或不生长的菌落，即阳性菌落，再以阳性菌落分别涂布zeocine含量500ug加， 1000ug砌l的ypd平板，以高浓度的抗生素筛选高拷贝的酵母工程菌，在含500ug ml高浓度抗生素平板上获得了15个转化子，取其中5个进行pcr扩增，有3个扩增产物与阳性对照相同，说明此酵母细胞中已含有s hbsag融合片段，其中之一命名为p - The recombinant plasimid ppic9k - pzp3 a - hcg p - ctp109 - 145 was transformed into electroporated pichia pastoris and screened the positive strains on md plates without histidine . multi - copy recombinants were screened on the g418 - ypd plates and then were incubated with the supernatant containing methanol . the recombinant pzp3 a - hcg p - ctp 109 - 145 protein were secreted in the supern atant and were verified
转化嗜甲醇酵母获得耐受4mg mlg418的菌株，经sds - page电泳和westernblotting检测表明其上清培养液中的蛋白可与猴抗pzp3抗体和兔抗hcg抗体发生特异性的反应。 - The objective of this research is to transform the cloned phytase gene into pichia pastoris in order to obtain high - yield phytase - producing strain and to optimize the engineered yeast media recipes for the scale - up production of phytase . main results of this research are as follow : 1 , xba i - linized recombinant plasmid ppic9k - phya was transformed into pichia pastoris by gene pulser . 98 positive transformants showing measurable phytase activities were screened on md plates and ypd plates containing g418 . they all grew quickly on both md plates and mm plates , which proved their phenotypes of methanol utilization were mut +
主要实验结查如下：西南农业大学硕士学位论文1 、用乃ai酶切携带植酸酶基因表达片段的重组质粒ppicgk夕句) a ，回收dna ，用genepulser电击转化毕赤酵母，涂布md平板，又用含不同浓度g418的ypd平板进行抗性筛选，得到98个可检测到植酸酶活力的阳性转化子，它们在md 、 mm平板上均表现快速生长，说明其甲醇利用表型是mut干。