thione meaning in Chinese
硫酮
Examples
- After the gst fusion proteins were processed by factor xa and were purified by gluta - thione - sepharose 4b affinity chromatography , the recombinant protein hbrp with high purification would be acquired . during the process of separation and purification , the every result which can show the location of the aim protein was monitored by 10 % sds - page . the gst fusion proteins acquired finally were examined and analyzed by the methods of western - blottin
原核表达系统具有表达效率高，操作方便等特点，但缺乏翻译? 3 ?后修饰，以pgex载体表达的gst hbrp融合蛋白，具有表达的蛋白稳定性好，在体外易于纯化等优点，表达的gst融合蛋白可用gluthioneseph删sc4b进一步纯化一factorxa酶切与测分离，从而大量获得hbrp重组蛋白，而且一次回收率大约在90以上。