teasy meaning in Chinese

爱戏弄人

Firstly , the eo genes of csfv - jl and csfv - ln9912 strains were amplified by rt - pcr and nested pcr and then sequenced after cloned into t easy vector . comparing the eo sequences with other strains eo by biosoftwares , dnastar5 . 0 and bioedit , the phylogenetic analyse revealed that all of the strains we have sequenced could be divided into groupl and groupii . two amino acid streches of 15 csfv strains eo , which form the rnase active site , and histidine residues essential for rnase catalysis in both ones were highly conserved . the eo protein propertys of antigen epitope , hydrophobicity , isoelectric point were also predicted by bioinformatic method
首先，采用rt - pcr和nest - pcr技术扩增了csfv - jl株和csfv - ln9912株eo基因，插入teasy载体后测序，应用dnastar5 . 0和bioedit软件将其与本实验室已测其它毒株和genebank中登录的csfv代表毒株eo基因序列进行比较，绘制了遗传进化树并预测了eo蛋白的抗原表位、疏水性、等电点等特性。