subculturing meaning in Chinese
传代培养
移种
Examples
- Effects of cell density on proliferation and osteogenic differentiation of human mesenchymal stem cells during subculturing in vitro
传代培养密度对人间充质干细胞增殖及成骨分化的影响 - The highest yield of protoplasts ( 4 . 2 x 106 / g f . wt . ) was obtained from 12 - day - old calli after subculturing on fresh medium
结果表明，用转代后生长12d的愈伤组织，分离的原生质体产率为4 - There are also different in inducing cluster buds from callus of clone gingkgo and its other tissues and organs , some callus can induce cluster buds , but others can not . differentiation rate from the callus of cotyledon can be up to 36 % and the number of cluster buds is positive related to the times of subculturing
但是不同器官诱导的愈伤组织对于丛生芽的诱导也是不同的，子叶诱导的愈伤组织的分化率较高，有36左右，并且继代次数的增加也能够增加分化的几率 - The apoptosis in the passage cells was detected by using flow cytometry . it was fmded that the apoptosis of passage 12 cells was not obvious . cells were subcultured to sixteenth passage . it proved that the subculturing of porcine ear skin fibroblasts was stable by using this kind of method combining trypsin cold treatment with trypsin heat treatment
在进行传代培养时，利用了流式细胞仪检测了培养细胞的凋亡情况，发现传至第12代的细胞仍无明显凋亡现象，细胞已传至16代以上，说明应用此方法可稳定地进行猪耳皮肤成纤维细胞的传代培养。 - Twenty one adventitious buds from 30 root segment cultures were obtained after subculturing on medium lacking growth regulator for 90 days . the effects of hormones 6 - ba and naa on the adventitious bud differentiation were investigated and optimized . the results were showed that 6 - ba had significant effect on bud differentiation and the optimized medium for adventitious bud differentiation was ms + l . 0mg / l naa + 0 . 5mg / l 6 - ba . there were 67 adventitious buds occurred from average 30 hairy root segment cultures
90d后统计不定芽数量，平均每30个根段培养物上可获得21个不定芽；添加0 . 5mg 16 - ba能显著地促进毛状根不定芽的分化，配合使用6 - ba和naa对毛状根不定芽的分化最为有效( ms + 1 . 0mg lnaa + 0 . 5mg l6 - ba ) ， 90d后统计，平均每30个根段培养物上可获得67个不定芽。