subclone meaning in Chinese
亚克隆
Examples
- To indetify the function of phaa , phab and phac , three genes were amplifed from the subclone of pseudomanas sp . producing phas ( poly - 3 - hydroxyalkanoates ) by pcr
为了分析验证phaa 、 phab和phac三个基因的生物学功能，本实验采用pcr技术，从合成pha的pseudomanassp - Then , we find out the write clones through the chemis hib . during the working , three probes which we used are from the published sequences of spidroin . after this , a genie library of subclone of dragline was constructed
然后我们从构建蛛丝蛋白的亚克隆文库着手，采用鸟枪法测序的策略，取从蜘蛛cosmid文库中经过证实的阳性克隆scos - ds1 ，大，小为40kb左右，用cai ， eco47 ，和hinf三种酶分别对其进行酶解分析。 - Phaa , phab and phac were amplifed from the subclone of pseudomanas sp . producing pha by pcr . the gel electrophoresis analysis showed that the molecular weights of cloned phaa , phab and phac were equal to fragment speculated from three orfs
利用所提取的开放阅读框架的序列设计三对引物，采用pcr技术，从合成pha的亚克隆片段中分离出phaa 、 phab和phac三个基因片段，经凝胶电泳分析表明，所克隆的三个基因分子量大小与推测的三个开放阅读框架中基因片段大小一致。