smai meaning in Chinese

斯马伊

Smai , kpni and sali flanking bamhi could be used to analyze the cloned fragment
为应用协心加双重报告基因研究根瘤菌的分子生物学积累了材料。
Vp3 gene of hl isolate of goose parvovirus derived from recombinant piasmid pproex htb - vp3 was subcloned into ecorl site of psy538 , and the reporter gene lacz with promoter pll was cloned into smai site of recombinant piasmid . both vp3 gene and lacz gene were cloned into noti site of psy681 , recombinan fpv transfer vector containing vp3 gene of gpv was obtained . the result is basis of construction of recombinant fpv expressing vp3 gene of gpv and gpv genetic engineering vaccine
本研究另从含有gpvh1分离株主要结构蛋白vp3基因的重组质粒pproexhtb - vp3中切取gpvh1株vp3基因片段，将其亚克隆于psy538的ecori位点，然后将带有痘苗病毒启动子p11的lacz报告基因平端克隆于上述重组子的smai位点，再用noti切下同时含有vp3基因和lacz报告基因的片段，再亚克隆于psy681的noti位点，构建出含有vp3基因的重组禽痘病毒转移载体，为构建表达vp3基因的重组禽痘病毒从而制备gpv基因工程疫苗奠定了基础。