rtpcr meaning in Chinese
逆转录pcr
Examples
- A complete cdna of 1047 bp was obtained by means of 5 ' - race ( 5 ' - rapid amplification of cdna end ) techniques using gene specific primer p1a5 - 1
Rtpcr结果证明它在苞片中强烈表达而在处于同时期的叶片中几乎检测不到其表达。 - Furthermore , we found the mutagenesis relies on the alteration on gene expression profile induced by mnng treatment ; and also , the expression of dna polymerase p ( pol p ) was proved increased after mnng treatment
这种不依赖于dna损伤的非定标性突变随后被证明依赖于mn ’ ng引起的细胞内基因表达的变化。进一步我们用rtpcr技术证明dna聚合酶e （ p 。 lp ）的表达在mn ’ ng处理后显著升高。 - Northern blot and rt - pcr analysis show that sh2a gene is ubiquitously expressed in many tissues with three transcripts . the aberrant expression of sh2a gene in some cancers was found . it suggests that sh2a gene relates to tumors
Rtpcr及northern印迹杂交发现sh人在多种组织中有表?二?达，有3个转录本，而且在癌及癌旁组织的比较中发现，肿瘤组织中表达较高，初步证明与肿瘤的发生有关。 - Normal tissues and tumor tissues methods a gene was cloned by exon trapping and exon linking technique . homologous analysis of the gene was researched by blast . then we explored the gene expression pattern in various human tissues by northern blot and rt - pcr
正常组织及肿瘤组织实验方法利用捕获的外显于进行blast同源性分析，之后采用外显于拼接技术克隆新基因，利用rtpcr及northern印迹杂交检测该基因的组织表达情况，同时以p 《 ctin作内对照。 - Thl2 gene was finally amplified by pcr with template genomic dna and cdna from roots , leaves , petals , stigmas and ovaries during bud stage in brassica oleracea l . ( 200110197 ) and brassica napus l . ( y578 - 2 ) . the dna sequencing result shows that the genomic gene of thl2 is approximately 900 bp from brassica oleracea l . in contrast to that of 850 bp from brassica napus l .
采用阿r和rtpcr技术，从甘蓝和油菜基因组和柱头总则a中扩增获得了thlz基因，基因组中得到的基因大约为900hp和850hp ， cdna大约为477hp 。在thlz基因中首次发现了两个内含于，并且甘蓝和油菜thlz基因的内含于序列同源性低于ich ，并且大小也有差异。