rhodococcus meaning in Chinese
红球菌属
Examples
- Therefore , research on cholesterol oxidase has become more and more important . a 1 . 6 kb dna fragment was amplified using pcr method from genomic dna of rhodococcus equi . 4 - 2g2
本研究采用pcr方法从分泌胆固醇氧化酶的马红球菌菌株4 ? 2g2基因组dna中，扩增出特异dna片段，该扩增片段全长约1 . 6kb 。 - Sequence analysis showed that , this fragment has a homology of 99 % to the previously reported choe from rhodococcus equi . after cloning and subcloning and three identical fragments were obtained from three independent pcr , lacking three continual bases ( ttc , encoding the pheamino acid ) compared with choe , thus it can be assumed that the new fragment , designated as choew , and choe belong to the same gene family , even it might be the natural mutation of choe
对片段进行克隆、亚克隆之后测序分析，发现与已克隆的来源于马红球菌的胆固醇氧化酶基因cboe有99的同源性；并且三次独立的pcr均得到相同的片段，所克隆的基因序列与choe相比，连续缺失三个碱基( ttc ) ，即相应的氨基酸序列缺失一个氨基酸( phe ) ，因此判断所克隆的基因片段与choe属同一基因家族或原有基因的天然突变体，命名为choew 。 - In this study , a new gene c / wew , encoding cholesterol oxidase , was isolated from rhodococcus equi . 4 - 2g2 found in china , which may be useful in clinical diagnosis healthy food and pest management in agriculture . in addition , the gene has been expressed successfully , the expression product has cholesterol oxidase activity , thus this work provided theoretical basis to the development of genetic engineering of cholesterol oxidase
本研究从我国自行分离的马红球菌4 ? 2g2菌株中分离到一种编码胆固醇氧化酶的新基因choew ，它将可应用于临床检测、保健食品和农业抗虫等领域；同时利用原核表达载体成功的在大肠杆菌中表达了目的基因，表现出胆固醇氧化酶酶活，为胆固醇氧化酶的基因工程利用开发作了一定的理论实验基础研究。