postinfection meaning in Chinese
传染病后的
感染后
Examples
- Some blastspores even can expanded , germinated as time went on . the cockroach restrained the blastospores by phagocytosing and nodulation , and many of them melanized 5d postinfection
注射诱导后血淋巴蛋白质最明显的变化是一些原有蛋白质浓度发生改变，如88k蛋白质显著增加，而42k蛋白质含量减少等。 - The infected cells were passaged so that the virus could be isolated . cpe were observed from passage 2 to passage 9 postinfection . the cultured virus supernatants were harvested when cpe were observed in 3 / 4 infected cells
接种后的胚肺细胞从第二代到第九代均出现细胞病变，当接毒细胞有3 4出现细胞病变时，收集培养的细胞，制作超薄切片进行电镜观察，发现了病毒样粒子。 - In order to get the soluble recombinant eo protein and inspect the protein expression status convinently , the egfp and eo gene were ligated into baculovirus transfer vector . with the co - transfecting sf9 cells of baculovirus recombinant transfer vector and linearized viral dna , and plaque purification in the posttransfection procedure , the pure recombinant baculovirus were harvested , which infected the sf9 cells for amplifying to generate a p - l stock . . in the meantime , the fluorescence microscopy detection indicated expressed egfp protein to confirm the heterogenous protein expression of recombinant baculovirus . the pi - stock from a pure plaque was used to generate a high liter p - 2 stock , which was determined in liter as 1 . 14 107pfu / ml by performing a plaque assay . when a volume of p - 2 stock infected the sf9 cells with moi 5 - 10 for expression , the strong fluorescence was obeserved on the day 3 of postinfection
此外，为了得到可溶性重组eo蛋白并便于观察重组蛋白的表达情况，我们将egfp基因与eo基因相连插入昆虫杆状病毒转移载体中，与线性杆状病毒dna共转染sf9细胞后通过噬斑纯化得到纯的重组杆状病毒，将其感染sf9细胞制备p1种子液，同时用荧光显微镜观察绿色荧光蛋白的表达情况剔除表达效果差的重组杆状病毒。再用p1种子液感染sf9细胞制备高效价的p2种子液。通过病毒液的梯度稀释和噬斑测定，确定p2种子液的病毒滴度达1 . 14 10 ~ 7pfu ml 。 - The expressed product was lysised with supersonic wave and purified by sds - page . elisa analysis revealed that the antigenicity of the vpi protein has been detected . the forth part - detection of aev - nh937 strain by in situ hybridization ( 1sh ) - probe was labelled with digoxigenin ( dig ) . then the probe hybrid with 5d , 10d , and 20d postinfection brain tissue of chicken . the results of the ish showed that the positive signal was found in 3 cases , while control group was negative . there has been a reasonable correlatien between this method and other detection test
经超声波裂解，用尿素溶解包涵体，电泳纯化后，利用elisa检测vp _ 1外壳蛋白，表明具有一定抗原性；第四部分：应用原位杂交检测aevi用dig标记的探针与sd 、 10d 、 20d的攻毒鸡脑组织杂交，来检测那v的rna 。