pmef meaning in Chinese
小鼠胚胎成纤维细胞
小鼠原代胚胎成纤维细胞
Examples
- We also investigated the factors including feeder layer cells , growth factors and complements , which may affect the cultivation and differentiation of these cells in vitro . the main contents as follows : ( 1 ) isolation and culture of mouse embryonic fibroblast ( pmef ) and preparation the feeder layers
主要内容包括: ( 1 )小鼠胚胎成纤维细胞( mef )的分离培养和饲养层细胞的制作以妊娠13 . 5天或14 16天的小鼠为材料,建立了mef分离培养体系。 - At primary culture , pgcs co - cultured with their gonadal stromall cells were well grown . when subculture , we used primary chicken embryonic fibroblast ( pcef ) , primary mice embryonic fibroblast ( pmef ) and snl cells to make feeder cells . forward research founded that the pcef cells were the most suitable for the growth of putative eg cells when having various cytokines
继代培养时,经过反复实验比较鸡胚原代成纤维细胞饲养层( pcef ) 、小鼠原代成纤维细胞饲养层( pmef ) 、 snl细胞饲养层等三种不同饲养层的作用效果,最终发现以鸡胚原代成纤维细胞制作饲养层同时添加各种生长因子最适合鸡类eg细胞的生长,但pcef与pmef之间的差异不显著。