phagemid meaning in Chinese
噬粒
Examples
- Through primary pcr . and finally the recombinant dna fragments were cloned into the phagemid pcantab 5e vector and introduced into
十五肽的接头连在一起，并克隆到噬菌质粒pcantab 5e中，电击转化大肠杆菌tg1 。 - The phagemid particles displaying functional scfvs were rescued by reinfection of helper phage m13ko7 , thus a murine antibody library was obtained
经辅助噬菌体m13ko7超感染回收全部重组噬菌体，此即噬菌体抗体库。 - A polypeptide with sequence of qkvdssggggs was designed to be a linker between c terminal of penicillin g acylase and n terminal of the coat protein . the ribosome binding site ( rbs sequence ) of psurfscript is also replaced by rbs sequence originating from bacillus subtilis . it was demonstrated that constructed phagemid can still express penicillin g acylase
将包含信号肽和琥珀终止密码子uag ( amber )的完整巨大芽孢杆菌青霉素g酰化酶基因克隆到噬菌粒psurfscript ，通过引入的11肽连接青霉素g酰化酶的c末端与噬菌体外壳蛋白gp3的n末端。 - It is firstly reported that the 85 . 6kda heterodimeric penicillin g acylase from bacillus megaterium was processed and functional displayed on the surface of phage fd . phagemid psurfscript was used to clone full - lenghth gene with amber stop codon of penicillin g acylase ( signal peptide - subunit - interaal peptide - subunit ) from bacillus megaterium
革兰氏阴性菌alcaligenesfaecalis的pga已在噬菌体表面获得展示，并证实有活力，革兰氏阳性菌来源的pga是否能在噬菌体表面成功展示，迄今尚未见报道。