pcr引物 meaning in Chinese
pcr primer
Examples
- According to the nucleotide sequence of selected antigen epitope , pcr primers supplemented with the ecor i and sal i sites were designed
根据选出的抗原表位区的核酸序列设计pcr引物，并于引物末端添加ecor和sa11酶切位点。 - Using the tpsl gene digested form the prokaryotic expressing vector as template , according to the published sequence , pcr primers of tpsl gene was designed , 1500bp fragment of tpsl was generated
以从t - vector中酶切得到的tps1为模板，根据已知序列设计pcr引物，扩增海藻糖- 6 -磷酸合成酶基因( tps1 ) 。 - The results indicated that the pcr primers designed could distinguish between marine bacteria and terrestrial bacteria , and could be applied in distinguishing the psychrophiles . sixteen strains which could produce cold - active protease and chitinase were screened by selective medium and
初步的分析表明，所设计的pcr引物能够较好地区分海洋性细菌和陆源性细菌，并且可以用于嗜冷海洋细菌的区分。 - Compared with the referred rab3a , the amplified rab3a beard five nonsense nucleotide mutations , but the deduced amino acid sequence from the nucleotide sequence were completely the same as the referred rab3a protein , which demonstrated that the cloned placenta rab3a was suitable for further study
与pcr引物设计的参照rab3a比较有5个核苷酸变异，与翻译的氨基酸序列完全一致。由此表明本实验获得的rab3a cdna可用于进一步研究。 - Genes coding mature peptide of igfs were achieved by pcr using another pair of oligo - nucleotide primers to induce to the suitable restriction enzyme site , and the igf - i product of pcr contains 230 base pairs . igf - ii contains 219 base pairs . 3
各另外设计一对特异性pcr引物，导入适当限制性内切酶切点，以上述连有目的基因的克隆载体为模板，采用pcr方法扩增基因片段，获得长度约230bp的igf -和219bp的igf -成熟肽基因序列。