pachia meaning in Chinese
帕恰
Examples
- The deleted mutant pap gene was also cloned into yeast secreted expression ppic9k vector to form ppic9k ~ 3 , then the vector was transferred into pachia pastoris gs115 strain . the specific expression protein was secreted into the medium after inducing with methanol and the protein amount reached about 50 - 60 u g per millilitre measured by uv - absorbed methods in the supernatant of the medium via high density fermentation . sds - page results showed that there was one protein band in the gel which molecular weight was about 34ku
将缺失型pap基因克隆于酵母分泌型表达载体ppicgk构成重组载体,然后导入毕赤酵母( p8chianastoris )菌株gslls细胞中,在甲醇的诱导下,经过酵母高密度发酵进行pap的表达,经sds page分析,结果表明,在培养基上清液中含有一明显的特异性蛋臼条带,大小为34ku ,经western blotting分析,该蛋白与法国pap抗血清有特异性反应,体外活性检测表明该蛋白对tmv的侵染性具有高度的抑制性,说明该pap基因在毕赤酵母gs中也得到了正确表达。