n端融合 meaning in Chinese
n-terminal fusion
Examples
- The pet30 a + prokatyotic expression vector with a insertion fragment of fad2 cdna coding sequence is constructed to express a fusion fad2 protein with a n - terminal s - tag . with this tag , the protein is easy to detect and purify
以pcr及酶切连接的方法构建一个含有fad2cdna编码序列的原核表达载体，此蛋白的n端融合有s - tag标签，便于对表达的蛋白进行southern印迹检测及纯化。 - Construction and expression of tandem multi - copy gene edcoding ca ( 1 - 8 ) me ( 1 - 10 ) hybrid fused acidic peptide to improve antimicrobial peptide ' s expression , a novel mass - production method is proposed . it is based on the neutralization of the positive charges of antimicrobial peptide by fusing to an acidic peptide to avoid the lethal effect of the expressed peptide on the host cells
为提高抗菌肽的表达，设计在抗菌肽基因的n端融合一段编码酸性短肽的片段作为对抗菌肽前体的模拟，以减轻表达产物对宿主的毒性；同时通过含有酶切位点的接头将该融合肽基因以同向串连的方式连接成多拷贝基因。