nifa meaning in Chinese
阿伊阵
全国大学校际击剑协会
Examples
- It is used as the bait to screen azospirillum brasilense sp7 genomic plasmid library which was constructed by fusing 0 . 5 - 3kb fragments of a . brasilense sp7 genomic dna to the dna - activation domain in the pgad plasmid vectors
Brasilensesp7的nifa全序列构建在pgbd - c2载体上，得到重组质粒pgbd - nifa ，以其为诱饵，筛选sp7质粒基因组文库(构建在pgad系列质粒上) 。 - When cotransforming pgbd - nifa and ad fusion genomic library into saccharomyces cerevisiae pj69 - 4a , 109 candidates interacting with nifa had been selected by testing for the expression of the his3 , ade2 and lacz reporter genes
诱饵质粒和文库质粒共转化酿酒酵母( saccharomycescerevisiae ) pj69 - 4a ，通过检测报告基因his3 、 ade2及lacz的表达进行筛选，初筛得到109个阳性酵母菌落。 - 11 interacting candidates proved to be the positives after isolating the mixed plasmids from yeast and recotransforming each purified ad / library plasmid and pgbd - nifa into s . cerevisiae pj69 - 4a respectively . the genes of the 11 positives had been sequenced and 4 different gene fragments were obtained
分别对其中的ad / library质粒进行分离纯化，将每个纯化质粒再次与诱饵质粒共转化酵母pj69 - 4a进行验证，得到11个阳性克隆，测序结果显示其中有4个不同的基因片段，分别命名为s4 、 s35 、 s64和s65 。 - The research showed that their interactions in yeast two - hybrid system are still steady when the vector was exchanged , in contrast , the interaction was disappear when the reading frame of each positive had been changed . the four positives were subcloned into suicide plasmid so that gene mutant strains could be constructed via conjugation and homologous recombination
为了进一步验证这些克隆的编码产物与nifa蛋白的相互作用，将它们与nifa基因互换载体后共转化酵母，仍然可以激活三个报告基因的表达，而阳性克隆移码表达的重组质粒和pgbd - nifa的共转化物则不能在选择性平板上生长。