msha meaning in Chinese
安全卫生管理
安全卫生局
Examples
- The result showed that the homology rate of pila gene among the 5 avian pathogenic e . coli strains tested and one human e . coli were from 89 . 8 % to 91 . 1 % , and the homology rate of amino acid were from 88 . 5 % to 91 . 8 % . the homology rate of pila gene sequence among 5 avian pathogenic e . coli strains tested and avian pathogenic e . coli reported ( serotype o1 , o2 , o78 ) were from 87 . 8 % to 90 . 2 % , and the homology rate of amino acid were from 84 . 6 % to 91 . 2 % . there had homology in avian pathogenic e . coli . there had some common antigen side in type 1 pili of avian pathogenic e . coli
结果表明：运用msha法检测1型菌毛的检出率为80 ( 36 45 ) ， pcr法的检出率为95 . 5 ( 43 45 ) ， pcr方法用于1型菌毛的检测比msha更加敏感、快速、特异性强；选择5株优势血清型鸡源致病性大肠杆菌代表株( o _ ( 89 ) ， o _ ( 119 ) ， o _ ( 141 ) ， o _ ( 127 ) )的1型菌毛pila基因的pcr扩增片段经纯化后，分别定向克隆到puc18质粒的多克隆位点，构建了含有目的基因片段的克隆质粒，并转化到dh5株大肠杆菌载体菌中，筛选获得阳性克隆菌株。 - In this paper , 45 e . coli strains isolated from chicken farms in sichuan province were determined to be the pathogenic e . coli by animal test . type 1 pili of 45 strains isolated was detected by msha . the pila gene of 45 avian pathogenic e . coli strains were amplified by the polymerase chain reaction ( pcr ) with primers designed according to the sequence of the pila gene in genbank . results showed that pcr was more sensitive , faster and more characteristic than msha to detect type 1 pili
本研究将从四川规模化鸡场分离鉴定、经1日龄雏鸡致病性试验得到的鸡源致病性大肠杆菌45株，采用d -甘露糖敏感血凝试验( msha )检测1型菌毛，根据genbank中公布的人源大肠杆菌1型菌毛pila基因序列设计一对引物用pcr扩增鸡源致病性大肠杆菌1型菌毛pila基因。